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Isolation and characterization of dark fermentative hydrogen prod | 34287

Applied Microbiology: Open Access
Open Access

ISSN: 2471-9315

+44 1300 500008

Isolation and characterization of dark fermentative hydrogen producing bacteria from soil


4th World Congress and Expo on Applied Microbiology

September 19-21, 2016 Las Vegas, USA

Payal Mazumder

Tezpur University, India

Posters & Accepted Abstracts: Appli Micro Open Access

Abstract :

Hydrogen production by dark fermentation is a promising technique for energy generation because of high production rate and utilization of a wide variety of substrates. In this study hydrogen producing facultative anaerobic bacterial strains was isolated and identified. Their H2 production parameters were optimized using glucose as substrate under anaerobic condition. On the basis of 16 S rDNA gene sequencing, the strains were identified as Bacillus cereus (B-1), Staphylococcus sp. (B-6) and Enterobacter cloacae (DH-89). Batch experiments were conducted to study the effects of initial glucose concentration and pH on the production of hydrogen gas. The maximum hydrogen production was recorded with initial glucose concentration of 20 g/L in all the three bacterial strains. The H2 yield was obtained as 17.1 L/mol glucose, 13.32 L/mol glucose and 10.5 L/mol glucose for B-1, B-6, and DH- 89 respectively. The optimum pH for maximum H2 production was investigated and it was found that the optimum pH for strains Bacillus sp. and Staphylococcus sp. is 7.0. However in case of Enterobacter sp. the yield was increased to 29 L/mol glucose at pH 5.0. The ability of the strains for utilizing other monosaccharides and disaccharides were tested and it was observed that the strains are capable of utilizing other carbon sources as well. The strains showed positive test for catalase and lipase. B-1 and DH-89 showed positive test for cellulase. The present finding suggests that the isolated strains at their optimum condition can be used for fermentative hydrogen production from various waste materials.

Biography :

Payal Mazumder has completed her MSc from Tezpur University, India and currently pursuing her PhD. She has published one manuscript in an international conference and submitted two manuscripts for international journal which are under review.

Email: payal.spinnersend@gmail.com

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