alexa Isolation Of Aspergillus Flavus From Arachis Hypogea & Its Aflatoxin Production
ISSN: 2161-0517

Virology & Mycology
Open Access

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10th World Congress on Virology and Mycology
May 11-12, 2017 Singapore

Padma Singh
Gurukul Kangri University, India
Posters & Accepted Abstracts: Virol-mycol
DOI: 10.4172/2161-0517-C1-021
Abstract
Peanuts may be contaminated with the mold Aspergillus flavus which produces carcinogenic substance called aflatoxin. For these purpose fungus contaminants of peanut sample was isolated by direct plate method. Lactophenol cotton blue staining was used for the identification of A.flavus. Aspergillus flavus was inoculated into Czapek-Dox broth to measure its growth as well as aflatoxin production at different duration as 5, 10, 15 & 20 days. Then aflatoxin was detected by paper chromatography & on paper 3 spots were identified & aflatoxin production was further confirmed by Thin layer chromatography (TLC) where on TLC plate 2 spots were identified, out of which one was pink & the other was yellow which fluoresces green under UV-light & from standard table it was confirmed that it was Aflatoxin G2 &the other was Aflatoxin B2. ELISA method was also performed to assess the presence of aflatoxin level in culture metabolite & then absorbance of culture metabolite was taken & it was found to be low, as compared to standard values, this indicate that aflatoxin level was low in culture metabolite.
Keywords- Aspergillus flavus, Arachis hypogea, Aflatoxin, enzyme-linked immunosorbent assay (ELISA), thin layer chromatography (TLC)
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