alexa Isolation, Partial Purification And Antimicrobial Activity Of A Bacteriocin-like Compound Produced By Pseudomonas Spp. EMM-1
ISSN: 2471-9315

Applied Microbiology: Open Access
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12th International Congress on Microbial Interaction and Applications of Beneficial Microbes
July 17-18, 2017 Munich, Germany

Catherine Cesa-Luna, Antonino Baez-Rogelio, Yolanda Elizabeth Morales-Garcia, Rocio Perez-y-Terron, Jesus Muoz-Rojas and Veronica Quintero-Hernandez
Autonomous University of Puebla, Mexico
Posters & Accepted Abstracts: Appli Micro Open Access
DOI: 10.4172/2471-9315-C1-006
Abstract
Pseudomonas spp. EMM-1 is a Gram-negative bacterium isolated from contaminated soil highly competitive due to the production of one or more inhibitory substances. By testing in double-layer agar, its antimicrobial activity against diverse beneficial and pathogenic microorganism such as Bradyrhizobium, Azotobacter, Staphylococcus, Streptococcus, Klebsiella and Burkholderia, as well as phytopathogenic fungi as Pantoea and Fusarium has been demonstrated. Recently a crude extract was obtained by the “cold leaching methodology” and the physicochemical properties were partially characterized. It was reported a molecular mass between 3-10 kDa. The antimicrobial activity was active between -4ºC and 60ºC and at the pH range from 3 to 9; however, it loses its activity at 100ºC. When treated with a protease from Bacillus licheniformis it decreases 90% the activity of the crude extract. The activity due to the presence of siderophores was discarded. From this extract it was possible to purify an inhibitory substance by thin layer chromatography which inhibits some strains of the genus Streptococcus. Further purification by additional methodologies needs to be done.
Biography

Catherine Cesa-Luna completed her Bachelor’s degree in Clinical Chemistry in 2012 from Universidad Veracruzana, Mexico and obtained her Master’s degree in Microbiologic Sciences (Medical Microbiology) in 2016 from the Benemérita Universidad Autónoma de Puebla (Puebla, México). Currently, she is pursuing her PhD in Microbiology from Benemérita Universidad Autónoma de Puebla.

Email: [email protected]

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