alexa Isolation, Speciation And Antifungal Susceptibility Testing Of Candida Isolates From Various Clinical Specimens At A Tertiary Care Hospital, Nepal
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

International Conference on Medical and Clinical Microbiology
July 03-04, 2017 Bangkok, Thailand

Sundar Khadka
National Public Health Laboratory, Nepal
Posters & Accepted Abstracts: J Med Microb Diagn
DOI: 10.4172/2161-0703-C1-009
Abstract
Background: Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threating invasive diseases. Resistance to antifungal agents has increased during the last decades. Thus identification of candida up to species level and its antifungal susceptibility testing has paramount significance in the management of candidal infections. CHROM agar media can be reliably used for speciation of Candida isolates which helps to rapid identification of Candida species. The objective of the present study was to determine different species of Candida from various clinical specimens and to determine antifungal susceptibility pattern of candida species to four antifungal agents namely ketoconazole, fluconzole, miconazole, and clotrimazole. Methods: A total of 100 consecutive Candida isolates from various clinical samples were studied. Growths on Sabouraud’s Dextrose Agar were evaluated for colony appearance, macroscopic examination, gram staining, germ tube test and urea hydrolysis test. They were further processed for Candida speciation on CHROM agar. Different species of Candida were differentiated based on type of growth and color of isolates on CHROM agar media. Antifungal susceptibility testing was performed and interpreted for all the isolates using disc diffusion method as recommended by Clinical and Laboratory Standards Institute (CLSI) M44-A document. Results: Out of 100 Candida isolates, Candida albicans (56%) was the most common species. Among the non-albicans candida (NAC), Candida tropicalis (20%) was the commonest isolate followed by Candida glabrata (14%) and Candia krusei (10%) respectively. Overall susceptibility pattern of Candida species to clotrimazole found to be more sensitive (82%) followed by fluconazole (64%), miconazole (44%) respectively whereas ketoconazole was found to be more resistance (86%). Conclusions: Candida albicans was the predominant species responsible for various candidal infections. Among commonly used antifungal drugs clotrimazole, miconazole and fluconazole showed high sensitivity while ketoconazole was the least effective for both albicans and non-albicans group. CHROM agar is a simple, rapid & inexpensive method for identification of Candida species and is suitable for clinical laboratory with limited resources.
Biography

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords