Aged garlic extract (AGE) has been proven to exhibit antioxidant, hypolipidemic, hypoglycemic and antidiabetic properties.
However, its effect on diabetic nephropathy was unexplored. Therefore, the present study was designed to investigate the
renoprotective effect of AGE in streptozotocin-induced diabetic rats. Albino Wistar rats were induced with diabetes by a single
intraperitoneal injection of 45 mg/kg body weight of streptozotocin. Commercially available AGE was supplemented orally at a
dose of 500 mg/kg body weight/day. Aminoguanidine, which has been proven to be an anti-glycation agent is used as positive
control and was supplemented at a dose of 1 g/L in drinking water. The serum and urinary biochemical parameters were analyzed
in all the groups and at the end of 12 weeks follow up, the kidney histological examination was performed using H&E and
PAS staining. The diabetic rats showed a significant change in the urine (p< 0.001) and serum (p< 0.01) constituents such as
albumin, creatinine, urea nitrogen and glycated hemoglobin. In addition, the serum lipid profile of the diabetic rats were altered
significantly (p< 0.05) compared to that of the control rats. However, the diabetic rats supplemented with aged garlic extract
restored all these biochemical changes. The efficacy of the extract was substantiated by the histopathological changes in the
kidney. From our results, we conclude that aged garlic extract has the ability to ameliorate kidney damage in diabetic rats and the
renoprotective effect of AGE may be attributed to its anti-glycation and hypolipidemic activities.
Shiju TM has completed his B.Tech Biotechnology in Anna University. He is doing his Integrated Ph.D in VIT University, Vellore. He is receiving
fellowship from Council of Scientific and Industrial Research, India. He has done paper and poster presentations in Indian society of Nephrology and
in European Renal Association in the last year.
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