alexa Life In Nano Ice: Application Of CryoCrate C80EZ Medium For Cell And Tissue Cryopreservation
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

Joint Conference:3rd International Conference & Exhibition on TISSUE PRESERVATION AND BIOBANKING & 6th International Conference onTISSUE ENGINEERING AND REGENERATIVE MEDICINE
August 23-24, 2017 San Francisco, USA

Xu Han
University of Missouri, USA
ScientificTracks Abstracts: J Tissue Sci Eng
DOI: 10.4172/2157-7552-C1-033
Abstract
Traditional cryopreservation approaches are technically problematic in either producing large ice crystals (typically on um scale) associated with equilibrium freezing process or involving extremely high concentrations (40-60% v/v) of permeating cryoprotectants to achieve vitrification. Both also require the use of liquid nitrogen and expensive facilities for long-term storage or banking. With the support of NIH SBIR, Coulter Translational Partnership Program, and University of Missouri (MU) Fast-track Award, CryoCrate (a MU spin-off) has developed an innovative cryopreservation medium, trademarked as C80EZ, and opened the door for cryopreservation with both nano scale ice formation and long-term storage in regular deep freezers at approximately -80ºC. The base medium of C80EZ enables long-term storage of cells at -80ºC by preventing recrystallization and the efficiency has been demonstrated by previous publications, e.g. Sci Report 2016 (nature.com). The complete C80EZ medium enables unique nano scale ice formation and the efficiency tests have been performed on cell or tissue types that survive poorly from any traditional cryopreservation method, e.g. Human corneas, primary neurons, porcine and rodent spermatozoa, and cell types that cannot be stored in deep freezers for extended periods of time, e.g. prophetical blood mononuclear cells and mouse embryos. Significant improvement of cell post-thaw viabilities and functionalities has been observed for all above cell and tissues types. For E. coli competent cells (routinely stored in deep freezers), our preliminary data showed that the nano ice technology can remarkably increase post-thaw transformation efficiency. Currently, the C80EZ medium doesn’t improve survivability from cryopreservation for cell types with large volume of intracellular lipid components, e.g. porcine and fish embryos. The C80EZ medium is now commercially available, and the technology is patent pending (PCT filed) and exclusively licensed from MU to CryoCrate.
Biography

Xu Han has over 10 years of experience in the field for Cryopreservation. He is the Top Reviewer of the journal of Cryobiology, and a winner of NIH SBIR, Coulter Translational Partnership Program Award, and MU Faculty Innovation Award. He has endeavored in improving cryopreservation methods and devices. His expertise is also in the fields of Calorimetry, Numerical Simulation, Thermodynamics and Mechanics.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords