alexa Light Induced ATP Synthesis For Synthetic Biology Applications | 69063
ISSN: 2332-0737

Current Synthetic and Systems Biology
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3rd International Conference on Systems and Synthetic Biology

Christin Kleineberg
Max Planck Institute for Dynamics of Complex Technical Systems, Germany
Posters & Accepted Abstracts: Curr Synthetic Sys Biol
DOI: 10.4172/2332-0737-C1-009
Abstract
A unique feature of living cells is an ability to extract energy from their environment and to use this energy to carry out activities such as growth, movement or reproduction. In general, energy of nutrients (in cellular respiration) or light (in photosynthesis) is transformed via electron transfer chains (respiratory or photosynthetic) into a proton gradient across the cell membrane which is finally utilized for adenosine triphosphate (ATP) production. On the other hand, bacteriorhodopsin (bR) is widely known as proton pump machinery that transports the protons to the other side of the membrane due to light stimulation. Racker and Stoeckenius have studied a model system by combining bR and isolated ATP synthase in phospholipid vesicles for light induced ATP production. In order to design and construct a synthetic cell in the synthetic biology context, this kind of energy conversion “apparatus” should be well defined and optimized. Therefore, our idea is to build up a light-driven ATP generating “bioreactor” using the bottom up synthetic biology approach. In this context, functional parts (ATP-generating functional part and light-driven proton gradient functional part) are created, investigated, optimized separately and combined afterwards.
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