Reach Us +441414719275
Linolenic Acid And Thiazolidinediones Opens Up Switch On L6 Myoblast To Turn Into Adipoblast | 9079
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Linolenic acid and thiazolidinediones opens up switch on L6 myoblast to turn into adipoblast

International Conference on Animal & Dairy Sciences

N. K. Singh, S. Subi and A.M.M.T. Reza

AcceptedAbstracts: J Veterinar Sci Technolo

DOI: 10.4172/2157-7579.S1.004

Lately, it has been brought to light that if white adipose tissues could possibily be turned into brown fat it would certainly play critical role in curtailing down many disease pathogenesis. To understand what really happens on muscle cells when it turns to white adipose tissue, we investigated the adipogenic differentiation of mouse L6 myoblasts by combining two adipogenic inducers i.e. Linolenic acid (LA) and Thiazolidinedione (TZD). The L6 myoblast cells near confluence were selected to initiate the treatment with adipose induction mixture. While LA (100 uM) + TZD (10 uM) treatments were given after exposure with adipogenic induction mixture at every 48 hours and the samples were collected on d 0, 5 and 10. Significant adipogenesis were revealed with Oil-Red-O staining itself. However, higher genetic expressions of PPARγ, Adipo Q and LPL as well as the intense immunostaining of PPAR- Υ and CEBP- Υ further confirmed that initially when muscles gets converted it first turns into white adipose tissue. In conclusion, we realized that white adipose tissues formed during different pathological situation needs to be converted to brown adipose tissue to contain pathological process in the biological system.