Journal of Clinical & Experimental Pharmacology

Longgu (Fossilia Ossis Mastodi, dragon bone) changes the component profiles in Keishikaryukotsuboreito (Gui-Zhi-Jia-Long-Gu-Mu-Li-Tang)

5^th International Conference and Exhibition on Pharmacology and Ethnopharmacology

March 23-25, 2017 Orlando, USA

Kazuki Oguri and Kyoko Takahashi

Osaka University, Japan

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Longgu (Ryu-kotsu, Fossilia Ossis Mastodii, Os Draconis, or dragon bone) is the only fossil crude drug listed in the Japanese Pharmacopoeia, which defined as �??the ossified bone of large mammal." It is used as sedative component in Keishikaryukotsuboreito (KRB) formula in Kampo medicine (Traditional Japanese Medicine). In Japan, all longgu is purchased from People�??s Republic of China. Longgu resources are facing the threat of depletion. Effective countermeasures are urgently required. However, information is insufficient about role of longgu in Kampo formulas. We investigated the significance of longgu in KRB formula, which comprises seven crude drugs. We analyzed the components in KRB decoction using inductively coupled plasma mass spectrometry and gas chromatography. Slight inorganic components and no organic ones were detected from single decoction of longgu. The organic component profile of KRB decoction is different from that of longgu-free KRB decoction. The result of scanning electron microscopy with energy dispersive X-ray spectroscopy showed that decocted longgu captured organic materials at its uneven surface with small pores. We hypothesized that longgu adsorbs the components on its surface. The decocted longgu were submitted into solid state NMR and nitrogen adsorption measurements. The results of them showed that KRB-decocted longgu had smaller water content and specific surface area than raw and single decocted longgu, respectively. These results supported our hypothesis. We suggest recycling of longgu as one of the countermeasure for depletion though development of purging methods is needed.

Biography :

Kazuki Oguri is pursuing his PhD from Osaka University. He got a license for Japanese Pharmacist in 2013. His research focuses on longgu; one of the crude drugs used in Kampo medicine (Traditional Japanese Medicine). He has published three papers in journals.

Email: k-oguri@phs.osaka-u.ac.jp