alexa Low Androgen Action At A Critical Time-point In The Neonatal Period Down-regulates Biomarkers For Smooth Muscle Cell Differentiation Leading To Permanent Infertility
ISSN: 2161-1017

Endocrinology & Metabolic Syndrome
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

2nd International Conference on Endocrinology
October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore, USA

Lilian Okumu, Tim Braden, Liz Simon, and Hari Goyal
ScientificTracks Abstracts: Endocrinol Metab Synd
DOI: 10.4172/2161-1017.S1.005
Abstract
Previously we showed that exposure of neonatal rats to estrogens and anti-androgens at a critical time period induced penile mal-development, characterized by accumulation of fat cells and loss of smooth muscle cells in the corpus cavernosum. This study aimed to determine the cellular and molecular changes characterizing the above effects. Pups were treated daily with GnRH antagonist antide or diethylstilbestrol (DES), with or without androgen receptor (AR) agonist dihydrotestosterone (DHT) or estrogen receptor (ESR) antagonist ICI 182,780, for postnatal days 1-6. Tissues were collected at days 7, 10, 21 or adulthood. Testicular testosterone (T) was assayed and microarray performed on the 7-day old penile and testicular samples. Real-time-PCR was used to quantify expression of Ar, Esr1, Esr2, Pparg, Pde5a, Myh11 and Acta2. PDE5A, MYH11 and ACTA2 protein levels were determined by western blot and/or immunohistochemistry (IHC). Both estrogens and antiandrogens induced similar effects. Intratesticular T surge was decreased by 80-90%. The reduction was mitigated by ICI but not DHT co-administration. Microarray showed significant down-regulation of smooth muscle cell markers (Myh11, Myh7, Mylk, and Actg2) in the penis and steroidogenic enzymes (Hsd3β1, Cyp11α1, Cyp17α1 and Cyp2c22) and Star protein in the testis. Real-time PCR showed 50-70% reduction in Myh11 and Pde5a, which was confirmed by Western blots. ACTA2 IHC showed reduction in the number and size of corpus cavernosum?s sinusoids. Contrastingly, Pparg and Esr1 expression was up-regulated, while that for Esr2 and Ar was unaltered. These results provide evidence that estrogen-induced penile maldevelopment is mediated via ESR1 and AR pathways.
Biography
Lilian Okumu completed her PhD from University College Dublin, Ireland, and postdoctoral fellowship from Tuskegee University?s Center for Veterinary Medicine, Nursing and Allied Health in 2013. Currently, she is an assistant professor of Biology at Tuskegee University.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version