Necrotizing Enterocolitis (NEC) is a leading cause of morbidity and mortality among preterm neonates. To
identify subsets of neonates at high risk of NEC is a challenge. Local pro-infl
ammatory response of the host could be of relevance
in the still unclear pathogenesis of NEC. Macrophage migration inhibitory factor (MIF) is a pro-infl
implicated in the pathophysiology of infl
ammatory bowel diseases, such as chronic colitis and gastric infl
promoter contains a functionally relevant single nucleotide polymorphism (SNP) G→C at position -173. Th
e MIF -173 C allele
is associated with higher MIF expression
e aim of the study was to investigate whether the G/C polymorphism at -173 of the MIF promoter could be a risk factor
for NEC and mortality during NEC, in premature neonates.
Material and Methods:
In a retrospective study we enrolled 107, GA≤32 weeks, neonates admitted to NICU. Among them 41
ected by NEC (Bell�s stages I-II and III) and 66 were controls. MIF -173 genotyping was carried out by PCR and DHPLC
in all enrolled patients.
e frequency of the -173 G/C polymorphism and the distribution of -173 MIF genotype were similar in neonates with
NEC compared to controls. We did not observe a signifi
cant association between the -173G/C polymorphism and mortality.
e G/C polymorphism at -173 of the MIF promoter does not appear of relevance to identify subsets of neonates
at high risk of NEC and to predict mortality associated to NEC, in preterm neonates in NICU.
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