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Objective To construct an adenovirus expression vector encoding antimicrobial peptide PR39 , and to observe its expression
and antimicrobial activity in mouse macrophage RAW264.7 cells and lung. Methods Recombinant adenovirus vector pAd-
PR39 was used to infect mouse macrophage RAW264.7 cells and lungs and its antimicrobial activity against BCG was detcted.
The expression of PR39 gene in mouse macrophage RAW264.7 cells and lungs was detected by immunocytochemistry. Results
Recombinant adenovirus Ad-PR39 were infected into macrophage RAW264.7 cells and lung for stable or transient expression
of PR-39. PR-39 expression in macrophage cells was subsequently confirmed by immunocytochemistry and showed stronger
antibacterial ability against BCG than control group. Conclusion These results demonstrated that macrophage-specific expression
of antimicrobial peptide PR-39 in macrophages could inhibit the growth of intracellular BCG and indicated it to be a novel and
promising approach for the control of refractory intracellular infection.
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