alexa Magnetic Paper Based ELISA
ISSN: 2329-6798

Modern Chemistry & Applications
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Related Subjects

Share This Page

Additional Info

Loading
Loading Please wait..
 

5th Global Chemistry Congress
September 04-06, 2017 | London, UK

Greter Ortega and Edilso Reguera
Center for Applied Science and Advanced Technology (CICATA-Legaria), Mexico
Posters & Accepted Abstracts: Mod Chem Appl
DOI: 10.4172/2329-6798-C1-006
Abstract
In recent years, magnetic beads and nanoparticles (mainly magnetite) are reported, patented and commercialized as new platforms to improve ELISA (enzyme linked immunosorbent assay) performances. However, most of these technologies can be costly and impractical for the diagnosis of certain endemic and epidemic diseases in underdeveloped countries, where a considerable amount of patient samples must be studied. On the other hand, the most important application of paper (cellulose material) support of sensors by the scientific community has been the development of new paper-based analytical devices such as paper based ELISA assays. However, their sensitivity is currently more than ten times lower than that of traditional ELISA. This contribution reports a novel magnetic paper–based ELISA using core–shell [email protected] nanoparticles supported on a Whatman paper-like new solid immunoassay platform specifically for IgM-dengue antibodies recognition as the proof-of-concept target for antibodies isotype IgM detection. Affordable procedures to deposit magnetite nanoparticles on cellulose paper sheets (Whatman type-1 and ss903) and to conjugate such nanoparticles with antihuman-IgM antibodies using polydopamine as linker are reported. Structural features, magnetic behavior, coating homogeneity, and the nanoparticles/ linked antibodies ratio were determined. Additionally, magnetic paper – based ELISA for IgM-dengue antibodies detection provides a system with improved analytical response (two orders more sensitive with a 700 times lower limit of detection (LOD) than traditional ELISA or using magnetic beads without depositing), appropriate accuracy for real sample detection, low cost, easy manufacturing, and effortless and easy handling. Finally, a novel transducer based on quantum dots contained in polymeric capsules is proposed to be coupled in the magnetic paper–based ELISA for enhancing analytical response.
Biography

Greter Ortega completed her Bachelor of Science in Chemistry and Master of Science in Chemistry at University of Havana, Cuba in 2012 and 2015, respectively. At present, she is a graduate teaching Assistant in Department of Inorganic Chemistry, Faculty of Chemistry, University of Havana and a PhD student at Center for Applied Science and Advanced Technology of IPN, Legaria Unit, Mexico. She has published two research articles and participated in 10 international scientific meetings held in Cuba, Mexico and Spain and has done research on the synthesis, functionalization and the use of the novel properties of metal, magnetic and semiconductor nanoparticles to design nanostructured biosensors.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords