Proteome alterations observed upon functional activation or accompanying disease states of human cells may identify marker
proteins which may qualify as biomarkers. In order to record characteristic features we analyzed cultured and primary human
cells including leukocytes, fibroblasts, endothelial cells and epithelial cells at distinct functional states. Cells were characterized
by FACS analysis and processed according to standard operation procedures to obtain nuclear, cytoplasmic and secreted protein
fractions. Each protein fraction was fractionated by SDS-PAGE and gel slices were digested with trypsin. Peptides were separated
applying CHIP-HPLC and analyzed by peptide fragmentation using an iontrap mass spectrometer. Data were collected and
analyzed using the Griss Proteomics Database Engine. We observed that short term alterations of functional states such as
inflammatory activation are accompanied by most significant alterations in the secretome, while long term alterations such as
proliferation states are most clearly detected in the nuclear extract. Cytoplasmic proteins are best suitable to assess the cell type
and the metabolic state. These systematic analyses shall support semi-automated data interpretation algorithms suitable for cells
and tissue samples.
Christopher Gerner has completed his Ph.D in 1998 from the University of Vienna and postdoctoral studies from Trinity College in Dublin. From
2002-2012 he was head of the Clinical Proteomics Laboratories at the Medical University of Vienna. Since 2012 he is full professor for bioanalysis
at the University of Vienna.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals