Matrix resistance stress: A key parameter for immobilized cell growth regulation

6^th International Conference and Exhibition on Materials Science and Engineering

September 12-14, 2016 Atlanta, USA

Ivana Pajic-Lijakovic

University of Belgrade, Serbia

Posters & Accepted Abstracts: J Material Sci Eng

Abstract :

Irreversible nature of matrix structural changes around the immobilized cell aggregates caused by cell expansion is considered within the Ca-alginate microbeads. It is related to various effects: Cell-bulk surface effects (cell-polymer mechanical interactions) and cell surface-polymer surface effects (cell-polymer electrostatic interactions) at the bio-interface; polymer-bulk volume effects (polymer-polymer mechanical and electrostatic interactions) within the perturbed boundary layers around the cell aggregates; cumulative surface and volume effects within the parts of the microbead and; macroscopic effects within the microbead as a whole based on multi scale modeling approaches. Matrix irreversible structural changes within the boundary layers around the cell aggregates induces generation of the matrix resistance stress. The matrix stress is one of the key control parameters for the bioprocess optimization in order to achieve higher concentration of immobilized cells. The main rheological properties which gel matrix should satisfy are formulated in order to reduce the matrix resistance stress generated by compression within the boundary layers around the immobilized cell clusters caused by cell expansion. The stress reduction is prerequisite for achieving higher cell concentration within Ca-alginate beads. Two properties: the matrix viscoelasticity and the ability of stress relaxation have been proposed. In this work, we have connected these properties with loading conditions caused by cell rearrangement and growth occurred during repeated multi scale stress relaxation cycles. Herein, an attempt is made to discuss and connect various multi scale modeling approaches in order to shed further light to this complex course-consequence phenomenon.

Biography :

Email: iva@elab.tmf.bg.ac.rs