Journal of Pharmaceutical Care & Health Systems
Open Access
ISSN: 2376-0419
+44 1300 500008
ISSN: 2376-0419
+44 1300 500008
Jin Boo Jeong
Andong National University, South Korea
Posters & Accepted Abstracts: J Pharma Care Health Sys
Phlorofucofuroeckol A (PFF-A) as one of the phlorotannins found in the brown algae has been reported to exert anti-cancer property. However, the molecular mechanism for anti-cancer effect of PFF-A has not been known. Activating transcription factor 3 (ATF3) has been reported to be associated with apoptosis in colorectal cancer. The present study was performed to investigate the molecular mechanism by which PFF-A stimulates ATF3 expression and apoptosis in human colorectal cancer cells. PFF-A decreased the cell viability through an apoptosis in human colorectal cancer cells. PFF-A increased ATF3 expression through regulating transcriptional activity. The responsible cis-element for ATF3 transcriptional activation by PFF-A was CREB located between -147 to -85 of ATF3 promoter. Inhibitions of p38, JNK, GSK3�?² and I�?ºK-�?± blocked PFF-A-mediated ATF3 expression. ATF3 knockdown by ATF3 siRNA attenuated the cleavage of PARP by PFF-A, while ATF3 overexpression increased PFF-A-mediated cleaved PARP. These results suggest that PFF-A may exert anti-cancer property through inducing apoptosis through ATF3-mediated pathway in human colorectal cancer cells.
Email: jjb0403@anu.ac.kr