alexa Metabolic Profile Of Murine Melanoma Studied By Nuclear Magnetic Resonance
ISSN: 2153-0769

Metabolomics:Open Access
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd International Conference and Exhibition on Metabolomics & Systems Biology
March 24-26, 2014 Hilton San Antonio Airport, San Antonio, USA

Said Rahnamaye Rabbani
ScientificTracks Abstracts: Metabolomics
DOI: 10.4172/2153-0769.S1.024
Abstract
Using murine melanocytes, a new model of murine melanoma, called Tm-1, was produced by repeated cycles of cell disadhesion. In this strain, galectin-3, a multifunctional endogenous lectin that seems to act by modulating the mitochondrial response to different types of stress, is silenced. Through transfection of original Tm-1, we produced two new strains of melanomas, one expressing galectin-3, and the other not. In order to evaluate the changes in glucose metabolism induced by galectin-3, these cells were cultivated in different conditions of oxygen tension and glucose level. Approximately 107 cells were collected from each culture. The metabolic extraction protocol with methanol:water (1:1) was applied, each sample was sonicated five times, centrifuged and the supernatant was collected. These were lyophilized and then diluted in D 2 O for resonance experiments. The 29 absorption lines of each spectrum were integrated individually and used as input for principal component analyses and partial least squares discriminant analyses. The changes in concentration of certain metabolites were attributed to the impact of galectin-3 in the mitochondrial homeostasis process. Among all metabolites pointed out by analyses, the most relevant were lactate, free choline, GPC/PC, creatine, acetate, 3-hydroxybutyrate and taurine. It seems that galectin-3 acts in mitochondrial homeostasis only in the specific case where tumorigenic cells are exposed to stress, such as hypoxia. A probable explanation for the homeostasis relies on fact that cells expressing galectin-3 are able to remove from cellular environment those mitochondria that do not properly metabolize pyruvate received from the glycolytic pathway (?deficient? mitochondria).
Biography
Said Rahnamaye Rabbani graduated and obtained MSc in Physics. He got his D. Phil from Oxford University in Biophysics. He worked in several universities in Brazil and since 1988, he is the fellow of Sao Paulo University. Since 1999, he built and is actually coordinating the Magnetic Resonance Laboratory. He was the president of research committee of Physics Institute of USP. He acted as visiting scientist in National University of Cordoba, Atomic Center of Bariloche in Argentina and National University of San Antonio Abad of Cusco, Peru. He coordinated and acted as member of organizing committee of several national and international conferences.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords