Tomato, our major source of vitamins and anti-oxidants, represents an important market around the world. However,
tomato production is relentlessly challenged by environmental hazards, but also by changes in the consumer?s demand
for taste and nutritional value, thus requiring constant efforts to ameliorate varieties, but also cultural practice. A large body of
literature shows the importance of genetic factors in the control of tomato quality as well as environmental growth conditions.
Considering systems biology as a comprehensible quantitative analysis of the manner in which all the components of a biological
system interact functionally over time, we decide to use tomato plant-fruitas a biological system to studyhow this biological
system works under control of environmental factors.
Tomato (SolanumlycopersicumL., cv
) plants were grown under commercial culture conditions in a glass
greenhouse in Sainte Livrade (0.56 E 44.29 N) in South-West France and harvested during the summer season.
have been performed to characterise (i) the environmental conditions, (ii) the growth of the various plant organs, (iii) the
variation of the processes involved in carbon acquisition and allocation and (iv) the availability of endogenous resources. Major
environmental variables (temperature, light intensity, vapour pressure deficit, fruit temperature, etc.) have been recorded
throughout the whole plant growth.
To evaluate the biochemical fruit composition, metabolomics profiles were acquired by 1H
NMR, GC-MS, LC-MS-QTOF.
By using kinetic models describing metabolism (Poolman et al., 2004) as well as ecophysiological
models (G?nard et al., 2007), which enable the integration of environmental variables into the prediction (e.g., light, water and
temperature) we estimate roles played by primary metabolism in determining fruit performance, i.e. biomass production and
quality (sugar and organic acid contents).This presentation will focus on the metabolomics aspects of this project within the
overall context of an integrated functional genomics approach.
Dr. Rolin has completed his PhD in France and postdoctoral studies in Philadelphia at USDA. He is heading the ?Centre G?nomiqueFonctionnelle
Bordeaux? a federation of seven technological platforms, dedicated to the study of living organisms at a cellular and molecular scale in the
French Aquitaine Region. In 2002, he had set up and lead the Metabolome Facility in Bordeaux. D. Rolin isPresident of the R?seauFran?ais de
M?tabolomiqueetFluxomiquea French scientific society that aims at the promotion of Metabolomics &Fluxomics in France. D. Rolin is author of 63
research articles in peer-reviewed scientific journals.
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