Reach Us +1-504-608-2390
Metformin Inhibitory Function On Tumor Cell Induce By Hypoxia | 19729
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Clinical & Cellular Immunology

Z Safari, M H Seyedzadeh, R Safaralizadeh, Ayla Valinezhad Orang and G A Kardar
Accepted Abstracts: J Clin Cell Immunol
DOI: 10.4172/2155-9899.S1.019
Background: The formation of the hypoxic acidic in tumor is an important factor of invasion and metastasis. Although it has been reported that metformin acts as a proliferation inhibitor and apoptotic elevator in cancer cells, the role of metformin in low oxygen supplies has not been yet identified. In line with the differences in oxygen supplies in cancer cells compared to normal cells, in this current study we provided both hypoxia and normoxia condition in order to evaluate the actual anticancer effects of metformin on cancer cells. Material and method: Normal cells (HEK239) and cancer cells (MCF-7) in both hypoxia and normoxia condition were cultured and treated with different concentrations of metformin. Their proliferation, apoptosis, and necrosis rate assessed using MTT test and annexin V assay. The phosphorylation rate of S6K1 assessed using western blotting. Zymography and western blot were used to measure the expression levels of MMP-9. Results: Metformin inhibits proliferation more effectively in hypoxia condition compared to normoxia in cancer cells, while it has no significant difference between normoxia and hypoxia conditions in normal cells. Statistical analysis indicates that metformin causes an increase of apoptosis rate in cancer cells with hypoxia condition (p value <0.05), while there is no acceptable increase in normal cells. In addition metformin cause a significant decrease in S6K1 phosphorylation and activation in cancer cells under hypoxia condition compared to normoxia. But there was no significant difference between normoxia and hypoxia condition in normal cells. It also leads to a significant decrease in MMP-9 expression levels more effectively in hypoxia condition compared to normoxia. Conclusion: Our results indicate that in hypoxia condition metformin exerts its anti-cancerous function by inhibiting proliferation and tumor progression and inducing cell apoptosis more effectively than normoxia condition.
image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version