alexa MiR-9/MCPIP1 Axis Mediated Regulation Of IL-6 Expression In Osteoarthritis Chondrocytes | 17664
ISSN: 2161-0533

Orthopedic & Muscular System: Current Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Orthopedics & Rheumatology
July 28-30, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Tariq M Haqqi, Mohammad S Makki and Abdul Haseeb
ScientificTracks Abstracts: Orthop Muscul Syst
DOI: 10.4172/2161-0533.S1.016
Abstract
Background/Purpose: Post-transcriptional regulation of cytokine expression is important for maintaining tissue integrity. MCPIP1 was identified as a novel protein, which destabilizes inflammatory cytokines mRNAs via their 3? UTR. IL-6 has recently gained attention because of its high levels in synovial fluid in Osteoarthritis (OA) and ability to induce high levels of MMP-13 in OA. In the present study we determined whether MCPIP1 regulates IL-6 expression and evaluated the role of miR- 9/MCPIP1 axis in the regulation of IL-6 in human OA chondrocytes. Methods: Human chondrocytes were prepared from OA cartilage by the enzymatic digestion. TaqMan assays were used for gene expression analysisusing RNA isolated from cultured primary chondrocytes or from damaged or smooth regions of OA cartilage or RNA immunoprecipitation (RIP). RNA fluorescent in-situ hybridization (ISH) for IL-6 and MCPIP1 expression was performed using RNAScope. Transfection was done using Amaxa kit. Knockdown experiments were performed using Trisilencer-27 human siRNA. For RIP, lysates from IL-1 β -stimulated chondrocytes were incubated overnight with anti- MCPIP1 antibody or with isotype control IgG followed by RNA purification. Results: MCPIP1 expression was low in damaged cartilage compared to smooth cartilage while the expression of IL-6 was high in damaged cartilage and low in smooth cartilage, suggesting that lower expression of MCPIP1 may be contributing to the excessive expression of IL-6 in OA. Expression of miR-9 predicted by Target scan to bind the seed sequence in MCPIP1 mRNA was high in damaged cartilage compared to smooth cartilage and was also upregulated by IL-1 β in OA chondrocytes. Over expression of miR-9 mimic or inhibitor in OA chondrocytes altered the expression of MCPIP1 and IL-6. IL-1 β -mediated induction of IL-6 was initially low in OA chondrocytes but was significantly accelerated 8 h post-stimulation. On the other hand, expression of MCPIP1 was high initially in IL-1 β -stimulated OA chondrocytes but started to decline 8 h post-stimulation. Over expression of wild type MCPIP1, but not of mutant MCPIP1, in OA chondrocytes reduced the expression of IL-6 mRNA and protein significantly (p<0.05). Importantly siRNA-mediated knockdown of MCPIP1 elevated the IL-6 mRNA expression in OA chondrocytes. TaqMan analysis of the immunoprecipitated mRNAs showed that anti-MCPIP1 antibody pulled down larger amount of IL-6 mRNA than control IgG antibody did thus demonstrating the binding of MCPIP1 with IL-6 mRNA in OA chondrocytes. Conclusions: In this study for the first time expression of MCPIP1 and miR-9 in human OA cartilage and chondrocytes is shown. The data also demonstrate miR-9/MCPIP1/IL-6 interactions and provide evidence of miR-9/MCPIP1 axis as an important regulator of IL-6 expression in OA
Biography
Tariq M Haqqi completed his PhD from Aligarh Muslim University, India and postdoctoral studies from Marseille-Luminy, France and Mayo Clinic, Rochester, MN. He is Professor of Anatomy and Neurobiology at the Northeast Ohio Medical University, Rootstown, OH. He has published over 80 papers in highly reputed journals and has served as Editor-in-Chief and Editorial Board Member of reputed journals
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]micsonline.com

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version