alexa Mitochondrial Phosphoproteome
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd International Conference on Mass Spectrometry
October 10-11, 2016 Kuala Lumpur, Malaysia

Lemaire Claire
Institute of Integrative Biology of the Cell (I2BC), France
Keynote: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064.C1.021
Abstract
A large proportion of the proteome is subjected to post-translational modifications (PTMs), often associated with vital processes like cell cycle regulation, homeostasis, stress response, apoptosis, etc. Among the PTMs, phosphorylation is of key importance (at least 500 protein kinases and more than 100 phosphatases are predicted in the human genome) because, as a fast and reversible process, it potentially regulates almost all aspects of cell life. It is also known that abnormal phosphorylation events are associated with various cancers and neurodegenerative diseases. The steadily increasing number of known mitochondrial phosphoproteins, kinases and phosphatases in last few years suggests that reversible protein phosphorylation could be an important regulatory event in mitochondria that needs to be better understood. We have used two techniques to analyze protein phosphorylation state on isolated mitochondria. The first one relies on 2D BN SDS-PAGE electrophoresis associated to Pro-Q Diamond staining. The second one is quantitative and it uses multiplex stable isotope dimethyl labeling followed by fractionation by SCX and phosphopeptides enrichment by IMAC before LC-MS/MS analysis. This second approach allowed us to produce the first extensive study providing quantitative data on phosphosite responses to different carbon substrates in the yeast Saccharomyces cerevisiae (Renvoisé et al., 2014, J. Proteomics). It is an essential step forward in the analysis of regulation of mitochondrial proteins by phosphorylation.
Biography

Lemaire Claire is a Biochemist specialized in Membrane Proteins. She began her career in the Photosynthesis field on the assembly and regulation of Photosynthetic Complexes. She then joined the CNRS (French National Center for Scientific Research) where she acquired an expertise in the Respiratory Complexes. She has developed a research project with her group focusing on the regulation of OXPHOS complexes by phosphorylation.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords