alexa Molecular Characterization Of Enterohemorrhagic Escherichia Coli O157:H7 In Dairy Foods And Environments
ISSN: 2155-9600

Journal of Nutrition & Food Sciences
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

10th World Congress on Nutrition & Food Sciences
May 29-31, 2017 Osaka, Japan

Sang Don Ryu, Mi Ri Park and Sangnam Oh
Chonbuk National University, Korea
Posters & Accepted Abstracts: J Nutr Food Sci
DOI: 10.4172/2155-9600-C1-045
Abstract
The entry route of foodborne pathogens including EHEC O157:H7 from the dairy farm environment into raw milk and dairy foods may come from several different vectors, and influence on the human health. Here, we employed HT-29 intestine epithelial cells (IECs; in vitro) and C. elegans (in vivo) as host model systems and compared the specific host responses during EHEC O157:H7 infections using whole transcriptome analysis. To elucidate the immune pathways stimulated by EHEC O157, we employed quantitative real time-polymerase chain reaction (qRT-PCR), transgenic worms, and RNAi. After EHEC O157:H7 infections, the regulation of gene encoding detoxification process, stress response, immune response and oxidative stress-associated proteins were immediately remodeled. Among them, we showed that the genes encoding cytochrome P450 (CYP450) family were dramatically induced by >10- fold during infection of EHEC O157:H7 is common in both host models. Importantly, C. elegans mutants lacking CYP450 genes were highly susceptible to EHEC O157:H7 infections comparing to wild-type N2. Consistent with susceptibility tests, qRT-PCR results showed that CYP450 loss of function mutations significantly affected the transcriptional induction of antimicrobial peptide genes such as clec-60. Taken in their totality our results provide critical insights for host strategy to exclude EHEC O157:H7 pathogenesis in gastrointestinal (GI) tracts through cytochrome P450 family.
Biography

Sang Don Ryu has great experience on detection and characterization of foodborne pathogens in food microbiology research. In recent, he focused on the prevalence and molecular pathway of various foodborne pathogens including Listeria monocytogenes, Staphylococcus aureus, Camphylobacter jejuni, Salmonella typhumurium, and pathogenic E. coli isolated from dairy foods and environments. Especially, his outstanding results may help monitor and control the spread of bacterial species through the farm environment, which is a natural reservoir for these organisms.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords