alexa Molecular Characterization Of Mammalian Secretory Pathway
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th International Conference on Proteomics & Bioinformatics
August 04-06, 2014 Hilton-Chicago/Northbrook, Chicago, USA

Xuequn Chen
Keynote: J Proteomics Bioinform
DOI: 10.4172/0974-276X.S1.070
Abstract
The mammalian secretory pathway is critical forsecretion of hormones and enzymes and disruption of its homeostasis leads to human diseases including diabetes and pancreatitis. Applying quantitative proteomics in specialized pancreatic secretory cells, we aim to understand the regulation of the secretory pathway under different metabolic conditions. A molecular model of pancreatic zymogen granule (ZG) is critical for understanding its functions. After extensive characterizations of the components and topology of ZG membranes,here we report the determination of the absolute quantities of ZG proteinsusing GeLC-MRM and AQUA peptides. To determine thecopy numbers/ZG, atomic force microscopy was utilized to estimate the numbers and sizes of isolated ZGs. Pancreatic beta cell has well-developed endoplasmic reticulum (ER) for massive production of proinsulin. Perturbation of ER homeostasis contributes to diabetes. To characterize the molecular machinery for proinsulin biogenesis and ER homeostasis, MIN6 cell was used to purify rough ERby two different purification schemes, differential ultracentrifugation and step sucrose gradient. A total of 1696 proteins were identified in three experiments, among which 1279 proteins found in at least two experiments. This represents a comprehensive coverage of all known and many novel players in beta cell ER. To identify beta cell-specific ER proteins, tissue specific gene expression and Blast analysis were performed between our data and published ER proteomic data. Furthermore, targeted proteomics approach has been developed to validate mouse islets enriched proteins. iTRAQ-based proteomics analysis is also used to quantify the perturbation of beta cell ER homeostasis under different diabetes-causing conditions.
Biography
Xuequn Chen is currently an assistant professor in the School of Medicine at Wayne State University. He holds a PhD from the University of Michigan (2003) and did his postdoctoral work with Dr. Phil Andrews in the National Proteomics Center at Michigan. He has been conducting quantitative subsellular proteomics research in the mammalian secretory pathway and published his works in Molecular & Cellular Proteomics, Journal of Proteome Research, Proteomics and Journal of Proteomics
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords