Reach Us +441414719275
Molecular Characterization Of Some Virulence Genes Of Salmonella Enterica Serotype Sandow And Saintpaul Isolated From Environment Of Dairy Farms At Assiut Province, Egypt | 89870
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Molecular characterization of some virulence genes of Salmonella enterica serotype sandow and saintpaul isolated from environment of dairy farms at Assiut Province, Egypt

11th International Veterinary Congress

Eman Khalifa Sedeek and Sotohy Ahmed Sotohy Eman Khalifa Sedeek and Sotohy Ahmed Sotohy

Alexandria University, Egypt Assiut University, Egypt

ScientificTracks Abstracts: J Vet Sci Technol

DOI: 10.4172/2157-7579-C2-039

Dairy farm environment could become contaminated with Salmonella following outbreaks of illness, colonization of animals or by general contamination. The present study investigated a total number of 95 samples collected from three dairy farms in three different localities at Assiut Province including air (25), water (35) and manure (35) samples for detection of Salmonella species by bacteriological isolation, morphological, biochemical and serological identification followed by molecular characterization for the presence of six virulence genes; pefA (700bp), mgtC (677bp), stn (617bp), sopB (517bp), invA (284bp) and avrA (422bp) in Salmonella isolates. Bacteriological examination revealed the isolation of 3 (3.2%) Salmonella isolates from totally examined samples, which were identified serologically as one (2.9%) Salmonella enterica serotype sandow isolated from water and two Salmonella enterica serotype saintpaul from air and manure (one from each with 2.9% of each). Molecular characterization of the three Salmonella isolates revealed that all six tested virulence genes; except sopB (517bp); presented in Salmonella enterica serotype sandow isolated from water, while all 6 tested virulence genes found in Salmonella enterica serotype saintpaul isolated from manure and found that invA (284bp) was the only virulence gene located in Salmonella enterica serotype saintpaul isolated from air. This study highlighted on the potential sources for Salmonella contamination moreover the epidemiology of salmonellosis in dairy farms that necessitate following of strict healthful measures to reduce the risk of Salmonella infection that still constitutes a significant world zoonosis particularly through contamination of dairy farm environments that contribute to extend the recycling of Salmonella that considered the most vital sources of animal and human infection with Salmonella.

Eman Khalifa Sedeek is an Acting Dean of Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch; Assistant Professor of Microbiology and Immunology; Manager of Information Technology (IT Unit). She has her expertise in Biotechnology, Food Microbiology, Animal Microbiology and Molecular diagnostic Microbiology. She has the first record in Egypt and worldwide for isolation and identification of Trichoderma pseudokoningii Rifai from Egyptian immunocompromised cattle with Mycobacterium bovis infection with international record from Mycological Center, Assiut University, and has been published in Nature Precedings.

E-mail: [email protected]