Molecular Diversity Of Microbes With Probable Degradative Genes In Agricultural Soil Contaminated With Bonny Light Crude Oil | 27255
Journal of Ecosystem & Ecography
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This study looked at the diversity of microorganisms persistent in agricultural soil sample polluted with Nigerian Bonny
light crude oil for four years with a view to ascertain the presence of microbes with probable degradative gene for crude
oil degradation and to confirm similarities in microbial identities. DNA from crude oil polluted agricultural soil sample was
extraction using ZYMO soil DNA extraction Kit. DNA sequencing was performed thereafter, by Next Generation Sequencing
Technique (NGST) using automated PCR cycle- Genome Sequencer? FLX System from 454 Life Sciences? and Roche Applied.
Sequence analysis and alignment was performed using Vecton NTI suite 9 and the resulting nucleotide sequences were
compared to sequences obtained from GenBank by BLASTx analysis using CLO Bio software as well as BLASTn using NCBI.
Molecular confirmation of similarities in microbial Identities was obtained by creating different dendrograms/ distance trees.
Gene sequencing carried out read 513 different nucleotide sequences. Every read was BLASTED and the result file saved.
Nine phylum with 47 corresponding culture-dependent species and 169 culture-independent bacteria clone was obtained. The
resultant tree however, showed various proteobacteria (a-, b-, d- and g- proteobacteria), bacteria, enterobacteria, firmicutes,
plantomycetes, acidobacteria group/fibrobacteres, Bacteriodetes/chlorobi Actinobacteria/high G+C and chloriflexi phyla. The
nucleotide sequences with no hit was sent to Genbank for asigning of ascension number. The isolation of the aforementioned
organisms from crude oil polluted agricultural soil left for four years, depict that the organism probably, have degradative genes
which aided their survival.
Ogbulie Toochukwu Ekwutosi has completed her Ph.D at the age of 34 years from Federal University of Technology Owerri (FUTO) Nigeria. She has also obtained
additional qualification as Registered Environmental Scientist by NREP, USA in 2008. She is a senior Lecturer in the Department of Biotechnology, FUTO, Nigeria.
She has published more than 30 papers in reputed journals and has been serving as an Editorial Board Member of Analele Universitatii din Oradea - Fascicula
Biologie, Romania. She is a member of societal bodies as Society for Applied Microbiology UK (SFAM), Graduate Women in Science GWIS USA, and Organization
for Women in Science for the Developing World (OWSD).
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