alexa Molecular Typing Of Escherichia Coli Harboring Genes Encoding Major Toxins (Stx1, Stx2, EaeA And Hly A) Isolated From Clinical Samples Using Random Amplified Polymorphic DNA(RAPD)*-PCR | 13718
ISSN: 2161-1009

Biochemistry & Analytical Biochemistry
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Conference and Exhibition on Biochemical & Molecular Engineering
October 07-08, 2013 Hilton San Antonio Airport, TX, USA

Ziad W. Jaradat
Accepted Abstracts: Biochem Anal Biochem
DOI: 10.4172/2161-1009.S1.003
Abstract
A total of 243 samples of E. coli were collected from both clinical sources and tested first for the presence of the four major virulence factors; stx1, stx2, eaeA and hlyA and were then subjected to genotyping using the RAPD-PCR methodology. Only 52 isolates contained toxin genes while the rest were non toxigenic. The majority of toxin producing isolates (45 out of 52) were isolated from urine while three isolates were from pus samples, 2 isolates were from eye swabs and one isolate from wound and one from aspirate samples. Interestingly, the only four isolates exhibited the four toxins originated from urine samples and could be of O157:H7 isolates. Other four isolates (3 from urine and one from pus) exhibited 2 toxins [entimine and hemolysin]. The rest of the toxin producing isolates exhibited only entimin eaeA gene. The isolates were typed based on the RAPD-PCR profiles followed by hierarchical cluster analysis to classify the isolates and understand the relationship among them. The dendrogram revealed 99 clusters implying a very high heterogeneity among the isolates. Since most of the isolates came from urine sample, the majority of the clusters contained only E. coli isolated from urine while the isolates obtained from none urine samples did not cluster together, they were rather scattered among the urine isolates. This study highlighted the high heterogeneity of the E. coli isolates form patient samples. In addition it appears that the majority of the isolates from urine samples might be commensal E. coli rather than pathogenic owing to the low numbers of these isolates producing toxins. The RAPD-PCR appeared to be a cheap and easy method for good discrimination among these none related E. coli isolates.
Biography
Ziad W. Jaradat completed his Ph.D. in Microbiology/Biotechnology in 1999 from University of Manitoba, Canada, and worked for 3 years at Purdue University as a postdoctoral research associate and as senior research scientist in SA Scientific for two years. He has moved to Jordan University of Science and Technology to assume a tenure track position in the Department of Biotechnology and Genetic Engineering. Currently he serves as the head of academic affairs at Al Ain Campus for Fatima College of Health Sciences. He has published more than 33 articles, book chapters and books and serving as an Associate Editor-in-Chief for the International Journal of Life Science and Medical Research and an Editorial Member on the Advances in Genetic Engineering/OMICS.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version