Nitrilase is one of the nitrile metabolizing enzymes that catalyzes the conversion of nitriles to corresponding acids. Based
on substrate specificities, nitrilases are classified as aliphatic and aromatic nitrilases. Number of nitrilases from microbial/
plant sources have been purified, characterized and their genes have been cloned and sequenced. In order to identify as to which
group the sequences of nitrilases belong to, two types of motifs i.e. aliphatic nitrilase motifs (MDMAl) and aromatic nitrilase
motifs (MDMAr) each with four motifs based on conserved catalytic triad (Glu-48, Lys-131, Cys-165) were designed. Conserved
regions were identified by performing multiple sequence alignment (MSA) using multiple EM for motif elicitation (MEME). The
manually designed motifs (MDM?s) were validated by ScanProsite and their presence was also confirmed by PRATT, Gblocks and
MEME. The ScanProsite search against the MDMAr exhibited some new sources of aromatic nitrilase from plant, animals and
microbes whereas MDMAl only exhibited nitrilase from microbes. Besides identifying unique motifs in nitrilase sequences and
to confirm their specificity towards nitriles, randomly selected sequences were validated or motifs were validated by analyzing
some important physiochemical parameters of corresponding nitrilases.
Nikhil Sharma is currently pursuing his Ph.D. in the Department of Biotechnology and his research entails the study of microbial enzyme both in vitro
and in silico. He has contributed chapters in books and research articles in peer review journals.
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