alexa Multifactorial Optimization Of Cell-cocooned Collagen-alginate Microspheres For Cardiac Tissue Engineering | 19970
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference on Tissue Science & Regenerative Medicine

Mehrdad Rafat
ScientificTracks Abstracts: J Tissue Sci Eng
DOI: 10.4172/2157-7552.S1.014
Abstract
Summary: Tissue-specific delivery of stem cells holds the potential to regenerate damaged heart tissue and to restore its functions after Myocardial Infarction. Here we describe a novel cell encapsulation technique and an optimization method toward delivery of stem cells to damaged heart tissue. Induced pluripotent stem cells (iPS) were encapsulated in hybrid spheres of collagen and alginate and characterized for cell viability and spheres physical properties. Background/Issue(s)/Objectives: Heart attack and failure is the number one cause of death in developed countries. Stem cell transplantation has been used as a promising therapy for heart disease. However, extensive cell attrition, and loss at the site of transplantation present a limit to therapeutic efficacy. We have hypothesized that cells viability, functionality, and target delivery will be enhanced by encapsulating the cells in hybrid collagen-alginate microspheres. Our main objectives are to develop cocooning techniques for effective delivery of viable stem cells to the damaged heart tissue and a mean to optimize microspheres properties with respect to size, shape, and encapsulated cells viability. Design/Method: Hybrid spheres of collagen and alginate were produced using a coaxial air jet flow technique. The method involves the gelation of a collagen-alginate-cell solution in a calcium chloride bath. Microspheres loaded with iPS were washed and transferred to a Petri dish containing culture medium and incubated at 37?C. Microspheres formation and morphology (shape and size) and viability of the cells were monitored and evaluated using light microscopy techniques. A 23 full multifactorial experimental design (MED) was employed to fabricate and test the spheres to determine optimum size, shape, and material composition. The effects of air flow rate, air gap distance and collagen to alginate ratio of the microspheres were studied. Results: The MED results demonstrated that the air flow rate had a large effect on size, e.g. the higher the flow rate the smaller the spheres. Collagen to alginate ratio and air gap did not have a significant impact on size. The shape was, however, influenced by all factors. These findings allow us to fine-tune spheres to meet the size and shape criteria for different cell types. Spontaneous degradation occurred faster in spheres with higher collagen content compared to samples with less collagen. iPS cells were successfully encapsulated within the spheres and found viable over a 5-day period in culture. Conclusions/Next steps: Preliminary results reveal that viable iPS cells could be encapsulated in hybrid collagen-alginate spheres. The impact of air flow rate and spheres composition on sphere?s size and shape were determined. The next steps will include extensive biological and physical characterizations of microspheres, i.e. collagenase biodegradation, release mechanism of the cells, and injection in animal models
Biography
Rafat received his Master?s and PhD degrees in Chemical and Biological Engineering from the University of Ottawa (Canada) with specialization in Biomaterials and Tissue Engineering in 2002 and 2007, respectively. He is the co-inventor of four patents and the senior author of several refereed publications. Rafat co-invented the first clinically-tested bioengineered cornea at the University of Ottawa Eye Institute. He has further developed the bioengineered cornea technology in his group at Link?ping University and taken it to the next level closer to commercialization. His research group?s interests are mainly focused on Biomaterials Engineering and development of cell-interactive tissue-engineered materials as implantable scaffolds for ocular and cardiovascular applications such as bioengineered corneas and cardiac patches and nano and microencapsulation systems for controlled delivery of stem cells, drugs, and proteins to target tissues. His career spans a broad range of professional experiences-from working as a researcher and lecturer in academia, a medical devices regulator at the Canadian Government, and a consultant to biotech industries. He recently received two prestigious awards/grants from the European Research Agency including FP7 Marie Curie Incoming Fellowship Award (IIF) and FP7 Career Integration Grant. He is also the Co-Founder and Director of R&D, LinkoCare LifeSciences AB (Ltd.), a spinoff of Link?ping University focused on commercialization of bioengineered corneas and other bioengineered tissues
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

bornova escort

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]csonline.com

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

https://www.gaziantepescort.info

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7