alexa Multi-locus Sequence Typing Of Campylobacter Jejuni Using A Novel Polymerase-chain-reaction-microsphere Method
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Congress on Bacteriology & Infectious Diseases
November 17-19, 2014 DoubleTree by Hilton Hotel Chicago-North Shore, USA

Ross T Barnard, Fang Liang, Lawrence Wong, Jillian Templeton and Pat Blackall
Posters: J Bacteriol Parasitol
DOI: 10.4172/2155-9597.S1.007
Abstract
Introduction: Campylobacter is a major cause of foodborne disease, with Campylobacter jejuni contributing more than 90% of reported cases. For diagnosis and monitoring of transmission, several genotyping methods have been developed, including multi-locus sequence typing (MLST). However, there is still a need for fast, cost effective methods for routine analysis. We developed a technique combining allele-specific PCR with a microsphere-flow cytometer system. Seven loci with singlenucleotide polymorphisms (SNPs) with the highest Simpson?s index of diversity (D) were selected from an MLST database. With these loci as a target, multiplex allele-specific PCR was conducted on microspheres in a single reaction and fluorescence signal was detected in a flow cytometer. The signal on the microspheres indicated which allele specific primer was consumed in the PCR. By this means all seven loci could be determined. This approach has a turnaround time of 4 h. Results: To date, the method has been tested with two strains of Campylobacter jejuni possessing known SNP patterns and six of seven loci have, at time of writing, been correctly determined for these strains, in a single PCR reaction. Conclusion: A new allele specific PCR-microsphere-based method for genotyping Campylobacter jejuni is under development. This approach will be useful in laboratories utilizing PCR and flow cytometers.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords