Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Maryam Sayah and Vahid Kiarostami
Islamic Azad University, Iran
Scientific Tracks Abstracts: J Chromatogr Sep Tech
Statement of the Problem: Polyacrylamide can be applied with multiple purposes in some industries such flocculants for clarification of potable water and treatment of municipal and industrial effluents and also used as grouting agents in the construction of drinking water reservoirs and wells, soil conditioning agents. Residual levels of unreacted acrylamide monomer in polyacrylamide can easily transfer to environmental and drinking water due to its water solubility and consequently causes, the most important source of water contamination. Acrylamide has been stated as carcinogenic to humans (Group 2A) with a lifetime cancer of 10-5 in 0.5 �?µg L-1 in drinking water. Methodology & Theoretical Orientation: A reliable, fast and safe method with easy operation for determination of acrylamide in water samples using a low density solvent based solvent-terminated dispersive liquid liquid microextraction (ST-DLLME) combined with GC-FID as a low cost detector which is available for most research laboratories has been developed and studied. Octanone and methanol were selected as the best extraction and dispersive solvents, respectively, using one factor at a time method. A central composite design (CCD) as a response surface methodology was used for multivariate optimization of the influences of other five factors (Volumes of extraction and dispersive solvents, PH, salt addition and extraction time) on the extraction efficiency. Under optimized condition (extraction solvent and its volume: Octanone, 175 �?µl; dispersive solvent and its volume: 683 �?µl; PH: 6.5; salt addition: 2.5% and extraction time: 4 min), the linear range was 0.3-550 ng ml�?�?�?¹ with detection (S/N=3) and determination (S/N=10) limits of 0.1 and 0.3 ng ml�?�?�?¹, respectively. The percentage recoveries of acrylamide from drinking and well water at spiking level of 0.5, 1 and 10 ng ml�?�?�?¹ were obtained in the range of 90.8-94.1%. Compared with the previously reported methods, the proposed ST-DLLME method indicates adequately figures of merit.
Maryam Sayah has completed her master's degree in 2016 from the Department of chemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran. Her research project under the supervision of Dr Vahid Kiarostami was on the Development of a low density Solvent Based Solvent-Terminated Dispersive Liquid Liquid Microextraction (ST-DLLME) for the Extraction of acrylamide in water sample using central composite design (CCD). She is interested in chromatography (GC and HPLC), microextraction and chemometric methods.