alexa Mycotoxin And Molecular Characterization Of Alternaria Species Contaminated Wheat Grains In Riyadh City, KSA
ISSN: 2161-0517

Virology & Mycology
Open Access

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10th World Congress on Virology and Mycology
May 11-12, 2017 Singapore

Rukaia M Gashgari, Youssuf A Gherbawy and Eman Al-Homaidi
King Abdulaziz University, Saudi Arabia
Qena University, Egypt
Princess Nora bint Abdulrahman University, Saudi Arabia
Posters & Accepted Abstracts: Virol-mycol
DOI: 10.4172/2161-0517-C1-021
Abstract
The mycological profile of the wheat grains selling in different markets in Riyadh, Saudi Arabia was studied with a focus on the different species of Alternaria. Fifty fungal species and one species variety belonged to 18 genus namely: Alternaria, Acremonium, Aspergillus, Cladosporium, Drechslera, Mycovellosiella, Mycelia sterilia, Fusarium, Embellisia, Penicillium, Phoma, Scytalidium, Stachybotrys, Stemphylium, Staphylotricum, Torula, Ulocladium and Xylohypha. Twenty-nine strains of Alternaria alternata were screened for their ability to produce altenuene, alternariol and alternariol monomethyl ether. Toxicity bioassay for the extracts of fungal isolates was conducted using Bacillus subtilus. Fungal extracts differed in their ability to inhibit the growth of bacteria before and after boiling. Random Amplified Polymorphic DNA (RAPD) technique using 5 different random primers (OPA03, OPA04, OPA10, V6 and M13) was used. All tested primers gave positive results with all tested isolates. The constructed dendrogram based on the results of 5 primers collectively showed that there is no correlation between DNA banding patterns and geographical distribution for the isolates.
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