Nonviral delivery systems are relatively easy to produce in the large scale, are
safe, and elicit a negligible immune response. Nanoparticles (NPs) off
promise as nonviral vectors as biocompatible and -degradable carriers of drugs
with targeting capacities to specifi
c cell sites by surface receptors of monoclonal
antibodies (mAbs). We present the eff
ect of Biodegradable NP systems on drug-
resistant B-chronic lymphocytic leukemia (B-CLL) cells in vitro, encapsulating the
drug, hydroxylchloroquine (HDQ), with NP surface coatings of specifi
Antibodies such as the anti human CD20 mAb, Rituximab. Our data indicate that these
nanoparticles with surface mAbs are suitable for selective drug delivery to B-CLL cells
and other lymphoma cell lines, producing a strong apoptotic eff
ect when loaded with
the lysosomotropic agent, HDQ. We also evaluated their toxic and therapeutic eff
on a Human/Mouse Model of Burkitt Lymphoma, adding this time Chlorambucil
to the system. We found that Rituximab-Chlorambucil-Hydroxychloroquine BNPs
induce a decrease in cell viability of malignant B cells in a dose-dependent manner.
e mediated cytotoxicity resulted from apoptosis, and was confi
rmed by monitoring
the B-CLL cells aft
er Annexin V/propidium iodide staining. Additional data revealed
that these BNPs were non toxic for healthy animals, and had prolonged an outstanding
survival in this mice model of human lymphoma.
rof. Eduardo Ra?l Mansilla is a Medical Doctor graduated from the School of Medicine of
the National University of La Plata, Argentina, in 1979. Senior Specialist in Internal Medicine.
Professor of Internal Medicine of the School of Medicine, National University of La Plata
Argentina. Former Vice Director Spanish Hospital La Plata Argentina. Vice President Argentine
Federation of Internal Medicine (FAMI). Former President, La Plata Society of Internal Medicine.
Former President, La Plata Society of Cancerology. Former Coordinator Argentine Diabetes
Society Immunology and Genetic Commeettee. Director, Laboratory of Tissue Engineering,
Regenerative Medicine and Cell Therapies of CUCAIBA La Plata Province of Buenos Aires
Ministry of Health Center of Procurement and Transplantation of Organs. First Prize Argentine
Haematology Society 2007 for the Research Paper ? Biodegradable Nanoparticles with
Chlorambucil and Hydroxychloroquine for the Treatment of Chronic Lymphocytic Leukemia?.
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