alexa New Frontiers In Structural Mass Spectrometry: Unraveling Dynamic Metabolomics
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

International Expo and Conference on Analytrix & HPLC
October 22-24, 2012 DoubleTree by Hilton Chicago-North Shore, USA

John A. McLean
KeyNote: J Chromat Separation Techniq
DOI: 10.4172/2157-7064.S1.001
Abstract
S ystems-wide analyses typically necessitate multi-dimensional datasets, which is oftentimes challenging to obtain. In many contemporary strategies, concentrating on only one or a handful of molecular targets limits the utility of the information for disgnostic and predictive purposes. Guided by the paradigm of systems biology, we have developed structural mass spectrometry technologies for the characterization of complex biological systems in for temporal and spatial dimensions. In parallel with these strategies, we have also developed new bioinformatics/biostatistical workflows to allow experiments to begin in an untargeted coarse grain hypothesis and funnel into increasingly targeted fine grain information. Metabolomics dynamics studies are performed through the integration of microfluidic cell trapping devices fluidly coupled with the structural mass spectrometer. Cells are trapped and kept viable by constant perfusion of cell culture media. By introducing a chemical perturbation to the media, the exometabolome is constantly readout in near real-time. Cell cycle experiments can be performed by perturbation and relaxation to the new cell state. By combining multiple cell trapping devices, paracrine signaling events can be examined with exquisite detail. Metabolomic mapping experiments are performed by integrating spatially-resolved MALDI ionization with structural mass spectrometry. In this technique, broad-scale metabolomics is performed where relative abundance is recorded at every spatial location and map reconstructions for every metabolite measured are made. The report will describe advances made in both temporal and spatial metabolomics for cell culture and intact tissue in areas ranging from cancer biology and parasitology to drug discovery and pathogen exposure.
Biography
ohn A. McLean is an Assistant Professor in the Department of Chemistry at Vanderbilt University and a faculty fellow in the Institute of Chemical Biology and Institute of Integrative Biosystems Research and Education. His recent awards include an Excellence in Teaching Award from the student members of the American Chemical Society, a Defense Threat Reduction Agency Research Award, an American Society for Mass Spectrometry Research Award, a Spectroscopy Society of Pittsburgh Award, an R&D 100 Award, and the Bunsen?Kirchhoff Prize from the GDCh. He has published over 60 manuscripts in the peer-reviewed literature, 8 book chapters, and 15 patents.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords