alexa Notch Signaling Promotes Differentiation To The Absorptive Cell Lineage After Massive Small Bowel Resection In A Rat Model
ISSN: 2161-0665

Pediatrics & Therapeutics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

JOINT EVENT on 11th International Conference on Clinical Pediatrics & 2nd International Conference on Pediatric Surgery
June 29- July 01, 2017 London, UK

Sukhotnik Igor
The Bruce Rappaport, Israel
Bnai Zion Medical Center, Israel
ScientificTracks Abstracts: Pediatr Ther
DOI: 10.4172/2161-0665-C1-039
Growing evidence suggests that Notch signaling promotes differentiation to the absorptive cell lineage rather than to the secretory cell lineage. The objective of this study was to determine the role of Notch signaling in cell proliferation in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats were randomly assigned to one of two experimental groups of 8 rats each: Sham rats underwent bowel transection and re-anastomosis, SBS- rats underwent 75% small bowel resection. Rats were sacrificed on day 14. Illumina's Digital Gene Expression (DGE) analysis was used to determine Notch signaling gene expression profiling. Notchrelated gene and protein expression were determined using Real Time PCR, Western blotting and immunohistochemistry. From 7 investigated Notch-related (by DGE analysis) genes, 6 genes were up-regulated in SBS vs control animals with a relative change in gene expression level of 20% or more. A significant up-regulation of Notch signaling related genes in resected animals was accompanied by a significant increase in Notch-1 protein levels (Western Blot) and a significant increase in NOTCH-1 and Hes-1 (target gene) positive cells (immunohistochemistry) compared to sham animals. Evaluation of cell differentiation has shown a strong increase in total number of absorptive cells (unchanged secretory cells) compared to control rats. In conclusion, two weeks after bowel resection in rats, stimulated Notch signaling directs crypt cells population towards absorptive progenitors.

Sukhotnik Igor has completed his MD from University of Medicine, Chernowitz, Ukraine. He is an Associate Professor at Ruth and Bruce Rappaport Faculty of Medicine and Head of the Department of Pediatric Surgery at Bnai Zion Medical Center, Haifa, Israel. He has published more than 130 papers in reputed journals, 11 chapters and one book. He is a member of the boards of the European Association of Pediatric Surgery and the International Pediatric Surgery Research Association and has been serving as an Editorial Board Member of repute.

Email: [email protected]

image PDF   |   image HTML
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version