alexa Novel Combinatorial Ligand For Antibody Separation: Peptide Ligand With Hydrophobic Charge-induction Group | 70441
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th World Congress on Chromatography

Shan-Jing Yao, Hui-Li Lu, Qi-Lei Zhang and Dong-Qiang in
Zhejiang University, China
Posters & Accepted Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-032
Abstract
Affinity biomimetic chromatography with peptide ligands is a novel technology for antibody purification, which has the advantages of high specificity, stable ligand structure and low cost. However, due to high affinity of specially-designed ligands for antibody molecule, harsh conditions often have to be used to elute the antibody from affinity resins, which certainly cause some damages on the antibody structure and biologic activity. Hydrophobic charge-induction chromatography (HCIC) is also one of new techniques for antibody purification with dual-mode ligands that combine hydrophobic and electrostatic interactions. Target proteins can be adsorbed on uncharged ligands at neutral pH via hydrophobic interactions, and eluted via electrostatic repulsion between target protein and the charged ligand at acidic condition. HCIC process has shown the advantages of salt-tolerance, mild elution and flexible CIP, but the binding selectivity was limited due to relatively simple chemical structure of HCIC ligands. In the present work, peptide ligands were combined with hydrophobic charge-induction groups to developed new type of combinatorial ligands for antibody separation. On one hand, the structure of peptide ligands was designed via the molecular simulation to ensure high affinity to antibody. On the other hand, the hydrophobic charge-induction groups were introduced to enhance the antibody binding and benefit the elution via electrostatic repulsion with the charge-induction effects. The combinatorial ligands were synthesized and coupled onto agarose beads to prepare new affinity resins. The adsorption behaviors of IgG were investigated, and typical pHdependent adsorption was found. New resins were evaluated with antibody purification from CHO cell culture broth, and high process efficiency was obtained. The results demonstrated that novel combinatorial ligands integrated high affinity of peptide ligand to improve binding selectivity and charge-induction effects for convenient elution, which is promising for cost-effective and largescale purification of antibodies.
Biography

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

kactakapaniyor.com

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

Taktube

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

porn sex

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

Gaziantep Escort

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

sikiş

[email protected]

1-702-714-7001Extn: 9037

instafollowers

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

mp3 indir

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

putlockers

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

seks

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version