Reach Us +1-504-608-2390
Novel Expression Of Schistosoma Mansoni Cathepsin L1 Suggesting Potential Role As A Diagnostic Marker For Human Schistosomiasis | 19678
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Clinical & Cellular Immunology

Ibrahim R B Aly, Eman E L-Ahwany, Enas Nweir, Shreif Edris and Suher Zad
Accepted Abstracts: J Clin Cell Immunol
DOI: 10.4172/2155-9899.S1.019
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin is a cysteine proteinase of the papain superfamily detected in their gut lumen. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L cDNA coding of Schistosoma mansoni was cloned providing high-level expression of heterologous proteins in Escherichia coli to produce recombinant schistosome cathepsin L with 597 bp. Our product had 99% similarity to a Schistosoma mansoni Puerto Rican preprocathepsin L (SmCL1) mRNA. The recombinant fusion protein was then analyzed by SDS-PAGE and Western blotting resulting in a molecular weight of approximately 31 kDa. The recombinant protein was expressed as inclusion bodies, purified under denaturing conditions. This recombinant protein could be recognized specifically by rabbit antiserum against Schistosoma adult worm antigen preparation (SWAP), showing that the expressed product possessed good antigenicity. The specificity and sensitivity of the purified recombinant cathepsin L against S. mansoni infected patients was assessed using ELISA. Sera from 97 S. mansoni infected patients, 30 patients infected with other helminthic infection and 30 healthy controls were collected. Anti-Cathepsin-L S. mansoni antibodies were detected with the sensitivity reached to 99% and specificity was 99.3 %. These results suggest that our product of S. mansoni cathepsin L might be considered as a suitable candidate for diagnosis of human schistosomiasis.
Hongyan Qin has completed her PhD from Fourth Military Medical University and Postdoctoral studies from Department of Immunology and Genomic Medicine in Graduate School of Medicine of Kyoto University. She is the Vice-Director of Department of Medical Genetics and Developmental Biology of Fourth Military Medical University. Until now she has published more than 20 papers in reputed international journals.
image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version