Applied Microbiology: Open Access
Open Access

Observing various actin structures using optical measurement without indicator

12^th International Congress on Microbial Interaction and Applications of Beneficial Microbes

July 17-18, 2017 Munich, Germany

Seong Chan Jun, Byeongho Park, Seunghan Jo, Seunghee Oh, Juhwan Lim and Hyungsuk Lee

Yonsei University South Korea

Posters & Accepted Abstracts: Appli Micro Open Access

Abstract :

The cytoskeleton controls the cell form and determines the mechanical strength of cells. It is made of three types of filaments such as microfilaments, microtubules, and intermediate filaments. The constituent of microfilament is actin which is the most abundant protein in cells. The globular actin is polymerized into the filamentous actin in the presence of adenosine triphosphate and divalent cations. The monitoring of these proteins is considerably important to investigate the mechanical and functional characteristics of cells. In current study, we demonstrate the optical measurement of actin polymers with different type of structures without any indicator. Our method has been employed to investigate the proteins. The absorption is useful to monitor the presence and concentration of sample in solution. Actin monomer, polymerized actin and bundle actin made by adding gelsolin and �?±-actinin provided clearly different result in photoluminescence. Each samples showed different emission feature for peak position and intensity. Using this in vitro result, we monitored the change of actin structure in usual cells. The photoluminescence based determination in vivo was significantly consistent with the result of dye based fluorescence images.

Biography :

Email: scj@yonsei.ac.kr