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|Esraa A El-Shafiee|
|Cairo University, Egypt|
|Posters & Accepted Abstracts: J Microb Biochem Technol|
|The current study was carried out to investigate the occurrence of verotoxigenic E. coli (VTEC) in different irrigation waters and tomato. For this purpose, twenty water samples (5 groundwater, 11 surface water and 4 wastewater) were collected from irrigation streams in three distinct locations. In addition, 137 tomatoes from the three farms were enrolled in the current study. Isolation of E. coli from water samples was performed using membrane filtration technique on Eosin Methylene Blue (EMB) medium while tomato samples were enriched in Tryptic Soya Broth and then streaked on EMB plates. E. coli was identified through colonial characters; Gram's stain and biochemical reactions while multiplex PCR was performed to identify VTEC. VTEC strains were found in 40% of water samples whereas 8.8% of the examined tomatoes were positive. The highest prevalence of VTEC was recorded in tomatoes irrigated with surface water in contrast to absence of VTEC in tomatoes irrigated with groundwater. Vt2 gene was the predominant gene among VTEC tomato isolates. In conclusion, detection of VTEC in irrigation water and tomato from the same farm highlights the potential role of irrigation water in the contamination of tomato which has a great public health implication.|
Dr. Esraa A El - Shafiee is currently working in Cairo University, Egypt.
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