Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
+44 1300 500008
ISSN: 2155-9880
+44 1300 500008
S Jamuna, R Ashok Kumar and S Niranjali Devaraj
University of Madras, India
Scientific Tracks Abstracts: J Clin Exp Cardiolog
Background: The regulation of lipid metabolism via autophagy in diabetes mellitus and obesity has been trending in the recent years. However, focus is required for atherosclerosis which develops due to lipid accumulation in the vessel walls. This study has been designed to explore the vital role of autophagy and its relationship with lipid metabolism in OxLDL induced foam cells. In this study, autophagy was induced by Oligomeric proanthocyanidins in OxLDL induced foam cells. Method: Foam cell formation was evaluated by Oil red O and Nile red staining. Accumulation of lipid droplets was confirmed by mRNA analysis of perilipin. Autophagic activity was determined by mRNA and protein expression. LC3b and p62 gene expression was examined by RT-PCR. The protein expression of LC3b, p62 and ATG5 was determined using Western blot analysis. Intracellular cholesterol and triglyceride levels in foam cells were quantitatively measured by enzymatic colorimetric assays. Lysosomal acid lipase levels were quantified spectroflourimetrically. Results: OxLDL induced macrophage derived foam cell showed increased accumulation of lipid droplets which was confirmed by Oil red O and Nile red staining. Oligomeric proanthocyanidins decrease intracellular cholesterol and triglyceride levels, increase lysosomal acid lipase activity, whereas the OxLDL induced foam cells exhibit increased accumulation of lipid droplets via overexpression of perilipin protein. Overexpression of LC3b and ATG5, and reduced expression of p62 are marker of autophagic flux. Conclusion: OxLDL-induced foam cells undergo autophagy upon treatment with Oligomeric proanthocyanidins.
S Jamuna has completed her MPhil in Biochemistry and joined PhD under the guidance of Dr. S Niranjali Devaraj, Department of Biochemistry, Guindy campus, University of Madras, India. She has an excellent knowledge of Bioinformatics and has published five papers in reputed journals.
Email: jamunasanker@gmail.com