Optical and electrical properties of bacteria were based on bio-detector for heavy metals (CdCl2 and NiCl2) pollutants

2^nd International Conference on Biomechanics, Bioengineering & Audiology

November 07-08, 2016 Las Vegas, USA

Maytham Abdala Ali Al-Shanawa, A Nabok, A Hashim, T Smith and S Forder

University of Basra, Iraq
Sheffield Hallam University, UK

Scientific Tracks Abstracts: J Bioengineer & Biomedical Sci

Abstract :

Environmental pollution can be defined as any discharge of material or energy into water, land and air, that causes adverse changes to the earth's ecological balance, or that lowers the quality of life. One of the most danger contaminations are the heavy metals, which are commonly referred to as trace metals; many trace metals are highly toxic to humans (e.g. Hg, Pb, Cd, Ni, As, Sn) and other living organisms in the environment. There are many techniques used for detection of heavy metals, for example; AAS, ICP-MS and Chromatography. In this project, the bio-cell sensor that included the microorganisms bacteria ( E. coli and D. radiodurans) was employed for detection of heavy metals, which is considered to be a cheap (cost effective), simple (easy to use), powerless (portable) and sensitive technique. Characterization of bacteria samples were carried out using a variety of experimental techniques, i.e. optical methods including optical density measurements, UV-Vis spectrophotometer, fluorescent microscopy and spectroscopy for studying light scattering in bacteria samples and electrical methods both DC and AC are used. The results of the optical methods appeared to be completely different of bacteria response and did not correlate with the (live/dead) bacteria ratio, which are due to the effect of (Cd2+, Ni2+) ions on light scattering. The electrical technique was used to study the effect of heavy metals (CaCl2 and CaCl2) on bacteria. The effect of metal salt appeared to be comparable on both E. coli and D. radiodurans bacteria. AC and DC properties of electrochemical solutions that contained E. coli and D. radiodurans bacteria were studied, and the results were compared to and normalized to the results of samples not mixed with metals. Comparative figures can be used to estimate metal concentration and the effect of metal on bacteria.

Biography :

Maytham Abdala Ali Al-Shanawa completed his PhD at Sheffield Hallam University (UK), under supervision of Professor Alexie Nabok in MERI. He has published more than seven papers; and attended about nine global conferences in (UK, France, Croatia, Jordan and Iraq).

Email: methem_aa@yahoo.com