alexa Optimization Of Extraction Protocol For Maximum Recovery Of Triol Ginsenoside From Cell Suspensions Of Panax Sikkimensis And P. Quinquefolium As Function Of Culture Age
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Conference on Pharmacognosy, Phytochemistry & Natural Products
October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Tanya Biswas, Shashi Raghuvanshi, A. K. Mathur and Archana Mathur
Posters: Biochem & Pharmacol
DOI: 10.4172/2167-0501.S1.003
Abstract
In the present study, efforts were undertaken to optimize a chemical extraction protocol for Ginseng bioactives: triol ginsenosides, from suspension cultures of two Panax spp., and their quantification. Methanol (100%, 70% and 30%), water (hot and cold), water saturated butanol and butanol saturated water were the chosen solvents for ultrasonication - assisted extraction from callus. Alternately, cells were refluxed with 100% methanol in a soxhlet arrangement for extraction. Butanolic extracts after downstream processing, were vacuum evaporated and redissolved in methanol. HPTLC quantification was using TLC silica gel 60 F 254 plates with chloroform:methanol:water; 13:7:2 and was scanned in a Camag TLC Scanner at 575 nm wavelength. It was observed that ginsenoside recovery was dependent on the choice of solvent rather than the method of extraction. Sonication ? assisted extraction with 100% methanol was found to be the best method for maximum recovery of the triol ginsenoside. This protocol was utilized to determine ginsenoside production kinetics in the two cell lines over a period of 50 days at 7-10 day time intervals. Both the lines were found to be dominantly producing triol ginsenosides: Re and Rg1 with a total recovery of 0.57% DW in P. quinquefolium and 0.732% DW in P. sikkimensis cultures. Rg2 was found to be produced in P. sikkimensis cultures in an age dependent manner irrespective of biomass changes. Detailed data will be highlighted during the presentation.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version