alexa Optimization Of Fermentation Conditions For Extracellular Production Of The Antineoplastic Enzyme, L-asparaginase By Novel Actinomycete Nocardiopsis Synnemasporogenes Sp. Nov. NEAE-85
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

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7th World Congress on Microbiology
November 28-29, 2016 Valencia, Spain

Noura El-Ahmady Ali El-Naggar and Hassan Moawad
Genetic Engineering and Biotechnology Research Institute, Egypt
National Research Center, Egypt
Posters & Accepted Abstracts: J Bacteriol Parasitol
DOI: 10.4172/2155-9597.C1.026
Abstract
The optimization of different fermentation conditions for L-asparaginase production by Nocardiopsis sp. NEAE-85 and its validation using Plackett-Burman experimental design and response surface methodology was carried out. 15 nutritional variables (temperature, pH, incubation time, inoculum size, inoculum age, agitation speed, dextrose, starch, L-asparagine, KNO3, yeast extract, K2HPO4, MgSO4.7H2O, NaCl and FeSO4. 7H2O) were screened using Plackett-Burman experimental design. The most positive significant independent variables affecting enzyme production (inoculum age, dextrose and L-asparagine) were further optimized by the central composite face-centered design-response surface methodology. An overall about 3 and a half-fold increase in L-asparaginase production was achieved in the optimized medium as compared with the un-optimized basal medium. As a result, a medium of the following formula is the optimum for producing an extracellular L-asparaginase in the culture filtrate of Nocardiopsis synnemasporogenes sp. nov., NEAE-85: Dextrose 4 g, starch 20 g, L-asparagine 10 g, KNO3 1 g, yeast extract 1 g, K2HPO4 1 g, MgSO4.7H2O 0.5 g, NaCl 0.5 g, FeSO4.7H2O 0.01 g, pH 7, temperature 37 °C, agitation speed 100 rpm/min, inoculum size 4% ,v/v, inoculum age 24 h and fermentation period 5 days.
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