Oral epithelial atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, peppers and hot meals, using the AgNOR and Papanicolaou staining techniques

2^nd International Conference on Cytopathology & Histopathology

August 10-12, 2016 Las Vegas, USA

Sayda Omer Mohammed Ali Ebnaof

Khartoum University, Sudan

Scientific Tracks Abstracts: J Cytol Histol

Abstract :

To evaluate cytological atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, hot meals and peppers using the AgNOR and Papanicolaou methods. A total of 180 individuals were evaluated, of which 60 were smokers, 34 were alcohol users, 52 were habitual peppers and hot meal (exposed) consumers, 24 were non-exposed and 10 were patients with Oral Squamous Cell Carcinoma, as an internal control. Cytological materials were obtained by brushing of buccal mucosa, on the border of the tongue and on the floor of the mouth and participants underwent the Papanicolaou test for cytological changes and AgNOR staining for evaluation of the mean number of AgNOR dots per nucleus. SPSS program was used to perform the Pearson chi-square test. The 95% confidence level and Odds Ratio were used. The features of cytological atypia were verified among 10 individuals, including 5 smokers, 2 alcohol users, 2 hot meals and peppers consumers and one non-exposed. For atypia among tobacco smokers, the adjusted Odds Ratio and the 95% CI were found to be 2. Increased keratinization was detected among 27 (45%) of the smokers (P<0.0001), 17 (32.7%) of the pepper and hot meals consumers (P<0.005), 4 (11.8%) of the alcohol consumers and among 2 (3.7%) of the non-exposed group. Statistical analyses revealed a greater mean number of AgNORs per nucleus in smokers (3.68) followed by (2.82) alcohol consumers, compared to the habitual peppers and hot meal consumers (2.28) and the non-exposed group (2.00). What�s more, 80% of the smears with cytological atypia were identified with 6 6 2 AgNOR mean count. The increase of the variables suggests that the evaluation of epithelial atypical changes in individuals exposed to smoking and alcohol carcinogens may be a useful screening tool. While hot meals and peppers did not seem to be a risk for oral mucosal proliferation, they increased the potency of keratinization and infection.

Biography :

Sayda Omer Mohammed Ali Ebnaof has completed her MSc in Molecular Medicine from Institute of Endemic Diseases, University of Khartoum in 2012. She is a Lecturer of Histopathology and Cytology at University of Khartoum, Sudan. She has published three papers in reputed journals.

Email: saydaomer1@hotmail.com