alexa Orexin Neuron Induction Through Epigenetics And Hexosamine Biosynthesis Pathway In Human And Mouse Pluripotent Stem Cells
ISSN: 2161-1017

Endocrinology & Metabolic Syndrome
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Conference on Endocrinology
October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore, USA

Kunio Shiota
ScientificTracks Abstracts: Endocrinol Metab Synd
DOI: 10.4172/2161-1017.S1.005
Abstract
Glucose is metabolized through several pathways; glycolysis, pentose phosphate pathway, and hexosamine biosynthesis pathway (HBP). Orexin system plays a central role of integration of sleep/wake in broad spectrum of neural-metabolic physiology. Orexin-A and -B, are produced from prepro-orexin encoded by HCRT/Hcrt gene. Despite enormous efforts, authentic methods successfully used to induce other hypothalamic peptide neurons could not generate the orexin neurons from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). We succeeded in generating functional orexin neurons from mouse ESCs and human iPSCs by adding ManNAc, an intermediate of HBP. The induced orexin neurons were sensitive to glucose, and possessed the ability to respond to neurotransmitter and peptides such as GABA, TRH, ghrelin, and leptin. CpGs of the Hcrt genome locus was hypomethylated, which was associated with H3/H4 higher acetylation. Concomitantly, histone acetyltransferase, p300, CBP and Mgea5 (O-GlcNAcase) were colocalized at the Hcrt gene locus in the orexin neurons. In the orexin non-expressing cells, hypoacetylation of H3/H4 and hyper O-GlcNAc modification were observed at the Hcrt gene locus, which were occupied with Ogt and Sirt1. Therefore, stream of HBP is sensed in the epigenetic mechanism to switch the Hcr gene from inactive state to active state. These results indicated that the multi-layer epigenetic regulation in the generation of orexin neurons. The established method will be useful for development of orexin neurons for regenerative medicine, and the induced orexin neurons will provide a strong tool for the development of medical applications for diseases related to the orexin system.
Biography
Kunio Shiota is a professor in the Laboratory of Cellular Biochemistry at The University of Tokyo. He obtained Bachelor of Science/Doctor of Veterinary Medicine from Miyazaki University in 1973, and his PhD from the University of Tokyo in 1979. He joined the Central Research Division of Takeda Chemical Industries Ltd., as a research fellow. After a decade of service at Takeda, he returned to The University of Tokyo in the Laboratory of Cellular Biochemistry and rose to his current position of Professor in 1988.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords