alexa Osteogenic Activity Of Constituents From Taiwan Native Plant | 70274
ISSN: 2329-6836

Natural Products Chemistry & Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

5th International Conference and Exhibition on Pharmacognosy, Phytochemistry & Natural Products
July 24-25, 2017 Melbourne, Australia

Yi-Tzu Lin, Yi-Wen Mao and Mei-Hsien Lee
Taipei Medical University, Taiwan
Posters & Accepted Abstracts: Nat Prod Chem Res
DOI: 10.4172/2329-6836-C1-017
Abstract
Osteoblasts and osteoclasts are two main cells of bone remodeling. While osteoblasts play an important role in bone formation via different signaling pathways, osteoclasts are responsible for bone resorption. Osteogenesis is characterized by the presence of a number of markers like: Alkaline Phosphatase (ALP) and type-I collagen at the differentiation stage of osteoblasts, while osteopontin and osteocalcin are actively expressed during mineralization (the mature stage). Uraria crinita (L.) Desv. ex DC. (Fabaceae) has been used for long as an herbal medicine to treat bone dysplasia in children in Taiwan and China. In the present study, we investigated the active constituents of the root from U. crinita by bio-guided isolation in primary human osteoblast (HOb) cells. Cell viability was determined using the WST-8 assay. Osteogenic activity was evaluated in HOb cells using ALP assay and Alizarin red S staining for mineralization. Gene expression was analyzed using real-time PCR. The results showed that 50% ethanolic extract of U. crinita roots increased ALP and mineralization activities. Six compounds were purified by chromatography and identified to be as: one phenolic acid, two flavone glycosides and three isoflavones from the active ethyl acetate fraction. Compound 4 (isoflavone) exhibited significantly increasing ALP and mineralization activities in HOb cells and it also up-regulated the osteogenesis-related gene expression. It may be considered to be the potential target for enhancing osteogenic activity in the future.
Biography

Yi-Tzu Lin has completed her Master’s degree in 2013 and currently pursuing PhD at Taipei Medical University, Taiwan. Her major research is isolation, identification and purification of the active compounds and investigation of their bioactivities as well as the related mechanisms.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version