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issue Engineering is a multidisciplinary science where a structural matrix is combined with cells to form a construct able
to promote regeneration of injured tissue. Bioactive glass foam (BG) produced by sol-gel is an well know osteoinductive
material. The human adipose stem cells (hASC) are capable of differentiating into osteoblasts. The use of bioreactors (BR) in
three-dimensional cell culture enables greater efficiency for cell nutrition and application of mechanical forces. The objective
of this study is to evaluate the osteogenic differentiation of hASC seeded on BG and cultured in three-dimensional perfusion
BR for the production of a functional construct for bone tissue engineering. The synthesis of BG resulted in an interconnected
network with pore size range 100-500μm and 88% porosity. The extraction and characterization of hASC were performed and a
new protocol for hASC cultivation in Leibovitz CO
independent medium (LEI) was developed for use in the perfusion BR. The
hASC cultivation on BG in the BR demonstrated a significant increase in cell proliferation and viability at 7 days of culture when
compared to static culture, ALP activity peak at 14 days and the immunofluorescence assay revealed expression of osteopontin
(OP), osteocalcin (OC), collagen type I and a gradual change in cell shape from spindle to cuboidal from 7 to 21 days of culture.
The PCR assay confirmed the expression of OP, OC and FA genes. In conclusion, it can be a promising strategy for cell culture to
obtain a functional construct for bone tissue engineering
Alexandra Rodrigues Pereira da Silva is graduated in Dentistry, Master and PhD in Materials Science. Currently she is a postdoctoral student at
Federal University of Minas Gerais in Brazil. She has published papers in renowned journals and recently is a reviewer of an international journal of
Biotechnology and Bioprocessing.
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