alexa Overexpression Of Na+/H+ Antiporter (NHX1) Gene To Enhance Salt Tolerance In Peanut (Arachis Hypogaea L.) | 17738
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

World Congress on Biotechnology

Venkatesh K, Chakrapani P and A Roja Rani
ScientificTracks Abstracts: JPB
DOI: 10.4172/2155-952X.1000001
Abstract
Groundnut (Arachis hypogaea L.) is an important commercial oil seed crop rich in high quality edible oil (56%), protein (30%) and carbohydrate (20%). India produces 8 million tonnes from 7.6 million hectares. Salinity is the major limiting factor in peanut crop productivity resulting in yield loss and reduced seed quality. Salt stress has adverse influence on water relations, photosynthesis, mineral nutrition, metabolism and growth. Mechanisms that confer salt tolerance vary with the plant species; however the basic strategy works towards the maintenance of Na+ homeostasis in the cytosol. One of the possible mechanisms is to increase the solute concentration in the vacuoles of the plant cells i.e. to compartmentalize sodium ions. The accumulation of sodium ions inside the vacuoles provides a 2-fold advantage (i) reducing toxic levels of sodium in cytosol and (ii) increasing the vacuolar osmotic potential with the concomitant generation of a more negative water potential that favors water uptake by the cell and better tissue water retention under high soil salinity. NHX1 gene was introduced into pCAMBIA1302 vector with hygromycin as the selection marker and GFP as the reporter gene, mobilized into Agrobacterium tumefaciens LBA 4404 and used for peanut transformation. The transformants were confirmed by PCR, Southern blotting and RT-PCR to confirm gene expression at the transcription level. Further evaluation of T1 and T2 plants in presence of 150 mM NaCl (including controls) for salt tolerance and ion analysis (Na+, K+ and Ca2+) in different parts of the transgenics. Our data demonstrate the potential of NHX1 for imparting enhanced salt tolerance capability to peanut varieties to grow in saline areas.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7