alexa Parameter Validation Of Analytical Methods Of Insecticide Residue Analyses In Foods Of Animal Origin, Feed And Water
ISSN: 2329-6631

Journal of Developing Drugs
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2nd International Summit on GMP, GCP & Quality Control
November 12-14, 2013 DoubleTree by Hilton Hotel Chicago-North Shore, IL, USA

Amsalu Debebe and S. Kuttalam
Accepted Abstracts: J Develop Drugs
DOI: 10.4172/2329-6631.S1.004
Abstract
The study was conducted to examine the interrelationship and coherence of analytical parameters in method validation. Seventeen organochlorines and fifteen organophosphates were studied in food matrices of animal origin: egg, milk, fish, mutton, feed and water under six analytical procedures. An auto-system gas chromatography equipped with 63Ni electron capture detector (ECD), flame thermo ionization detector (FTD), split injector and DB-5 capillary column with a 30 m length was used. The inner column diameter and film thickness were 0.25 mm and 0.25 μm, respectively, at a maximum usable temperature of 3250C. Recovery, sensitivity, linearity, precision and limits of detection (LOD) were tested. Compounds that fell out of the stipulated recovery, 70-120%, in a matrix have concurrently failed to meet the requirements for sensitivity (≥0.7), linearity (R2 >0.99) and precision (<0.2) in the same matrix. Highest LOD was recorded in those compounds and matrices. Different from the conventional point estimate, a new approach was introduced for setting upper and lower confidence limits of the LOD in quantitative analyses
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