alexa Pathogen Detection In Children With Both Mild And Severe Hand, Foot And Mouth Disease: New Factors Influencing Molecular Assay
ISSN: 1948-5964

Journal of Antivirals & Antiretrovirals
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

4th World Congress on Virology
October 06-08, 2014 Hilton San Antonio Airport, TX, USA

Xiu-Ying Zhao
Accepted Abstracts: J Antivir Antiretrovir
DOI: 10.4172/1948-5964.S1.022
The early diagnosis of Hand, foot and mouth disease (HFMD) relies on typical clinical manifestations, together with RTPCR assays from throat swab, herpes fluid or feces. This study included 1,104 clinically diagnosed HFMD patients. Swabs from 348 cases, with 115 mild cases and 233 severe cases were chosen for EV71, CA16 and 14 different respiratory pathogens (RPs) detection using PCR or RT-PCR. Furthermore, eight cytokines were detected to evaluate immune activation in HFMD patients, and serum IgM antibody to EV71 and CA16 from mild and severe patients was detected. Our results showed that factors, such as age, time of the year and infection of non-EV71/CA16 enteroviruses (EV) all contribute to the pathogen detection. Besides, the EV71/CA16-positive rate in mild cases was much higher than in severe cases (χ2 = 17.198, P = 0.000). The positive rate of RPs in the mild group was much higher than in the severe group (χ2 = 7.963, P = 0.005). No interference between RPs infection and the identification of EV. The presence of cytokines was shown to be significantly higher in the severe group than in the mild group. ELISA indicated the positive rate of EV71/CA16-IgM in severe cases were much higher than in mild cases (χ2 = 7.153, P=0.007).The lower positive rates of EV and RPs in severe HFMD cases by molecular method were found to be due to stronger immune response. Therefore, ELISA are essential supplement to PCR or RT-PCR in order to increase pathogen diagnosis in HFMD, especially in severe cases.
Xiuying Zhao had completed her Ph.D in 2005 from department of Microbiology, the University of HongKong. She has been worked in the field of experimental diagnosis of variety kinds of infectious diseases such as Hepatitis, AIDS, SARS, Flu, and HFMD. She is now work as director in department of clinical laboratory, Beijing Tsinghua hospital, Tsinghua University. She has published more than 50 papers in Chinese or in English.
image PDF   |   image HTML
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version