Pharmacokinetics Of Specific Probe Drugs Of CYP1A Enzyme: The Acetaminophen In Bactrian Camels | 72203
Journal of Veterinary Science & Technology
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Acetaminophen is a medication used to treat pain and fever, also is a specific probe substrate of CYP1A enzyme. The
pharmacokinetic characteristics of Acetaminophen in Bactrian camels were studied in this paper. The experimental
Bactrian camels were randomly divided into two groups: group probe drug only and group enzyme inhibitor plus probe drug,
respectively. A crossover design was carried out in two experimental periods following 15 days of drug clearance period.
Acetaminophen was intramuscularly injected to 6 female camels by 4 mg/kg in group probe drug only, and equal number of
female camels was intramuscularly administered by 4 mg/kg of Acetaminophen following 4 consecutive days of intramuscular
administration of lomefloxacin by 0.4 mg/kg in group enzyme inhibitor plus probe drug. And then the blood samples were
collected at different time intervals after administration of Acetaminophen, and the plasma was separated by centrifugation.
The plasma concentration of Acetaminophen was determined by high-performance liquid chromatography (HPLC) after
the samples' protein was precipitated by methanol directly, and the pharmacokinetic parameters of Acetaminophen were
calculated by WinNonLin 7.0. The pharmacokinetic parameters of Acetaminophen in group probe drug only and in group
enzyme inhibitor plus probe drug were as follow: the elimination half-life (T1/2) was 7.34±0.57 h and 8.98±0.31 h, the time to
peak concentration (Tmax) was 1.70±0.51 h and 0.833±0.31 h, the maximum plasma concentration of (Cmax) was 1.27±0.83 μg/
mL and 1.53±0.46 μg/mL, the area under the curve (AUC0-t) was 7.60±0.45 μg•h/mL and 10.71±0.25 μg•h/mL, the apparent
volume of distribution (Vd) was 3787.81±236.37 mL/kg and 2885.98 ±73.11 mL/kg, the clearance (CL) was 359.35±33.49
mL/h/kg and 222.75±8.79 mL/h/kg, and the mean residence time (MRT)was 10.35±0.84 h and 13.04±0.55 h, respectively.
Therefore, the Acetaminophen was rapidly absorbed and slowly eliminated by Bactrian camel, and the Bactrian camels' CYP1A
enzyme was significantly inhibited by lomefloxacin which can increase the T1/2, Cmax, AUC and MRT of Acetaminophen and
reduce the Tmax of Acetaminophen in Bactrian camel of China.
Guleng Amu is currently working as a Professor in College of Science, Inner Mongolia Agricultural University. Her research interests are mainly focused on Biomedical Engineering and Biological Physics.
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